Amidated neuropeptides by LifeTein for receptor binding study

Neuropeptides plays critical roles in every aspect of life. The honey bee short neuropeptide F (sNPF) was synthesized by LifeTein. These neuropeptides were amidated at the C terminus to mimic the native protein structure. The amidated neuropeptide ligands mutated to a different amino acid could not activate the receptor. The amidated Y amino acid is critical for receptor activation and decrease in intracellular cAMP production.

The result is supported by detection of a signature peptide fragment by MALDI-TOF MS and by functional cAMP assays.

(Reference: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109590)

Synthetic phospho-peptide for castor oil seed studies

Synthetic phospho-peptide from LifeTein were used for the castor oil seed studies. The purified phosphopeptides at Serine from LifeTein confirmed that the phosphorylation can protect sucrose synthase isozyme RcSUS1from proteolysis. The phosphorylation process is modulated by photosynthesis in leaves. Using polypeptides is an important tool to develop effective biotechnological strategies. reference: http://www.jbc.org/content/early/2014/10/13/jbc.M114.585554.short

A breakthrough for drug delivery into tumours using LifeTein cell penetrating peptides

Biotin labeled peptides were synthesized by LifeTein and conjugated with nanoparticles. A 6-amino-hexanoic linker was added between biotin and peptide to form a flexible space. It was found that a peptide designed for tumour-homing with a carboxy-terminal, basic sequence motif can act as cell penetration peptide to enter tumour tissue. This is a breakthrough for drug delivery into tumours. In addition, this neuropilins mediated endocytosis is distinct from known endocytic pathways. The nutrient regulation of the pathway is a new finding to use of the C-terminal, basic sequence motif  pathway in drug delivery.

http://www.lifetein.com/Quality-Peptides-For-Publications.html

How to dissolve peptides with organic solvents

Dissolving a hydrophobic peptide could be tricky. LifeTein's website offers free information for researchers on how to dissolve peptides in polar organic solvents such as DMSO or ethanol. The website on how to handle and dissolve peptides was cited in Extremophiles. Lifetein's guideline is helpful for researchers to understand the basic procedure on how to dissolve peptides using the best solvent without sacrifice the protein activity at non-aqueous environments.

The reference was published on Extremophiles: DOI: 10.1007/s00792-014-0683-4

Effect of organic solvents on the structure and activity of moderately halophilic Bacillus sp. EMB9 protease

R Sinha, SK Khare - Extremophiles

Read more on how to handle and dissolve peptides: http://www.lifetein.com/handling_and_storage_of_synthetic_peptides.html