Neuropeptides plays critical roles in every aspect of life. The honey bee short neuropeptide F (sNPF) was synthesized by LifeTein. These neuropeptides were amidated at the C terminus to mimic the native protein structure. The amidated neuropeptide ligands mutated to a different amino acid could not activate the receptor. The amidated Y amino acid is critical for receptor activation and decrease in intracellular cAMP production.
The result is supported by detection of a signature peptide fragment by MALDI-TOF MS and by functional cAMP assays.
(Reference: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109590)
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